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Also useful for receptor binding studies, screening inhibitors, and selectivity profiling. ×Recombinant human PDGFbb (HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent.
Similar approaches have shown that PDGF-BB can accelerate fracture healing in geriatric osteoporotic and diabetic rats , and enhance healing after bone distraction . Thus, there is accumulating evidence from preclinical human recombinant research that PDGF-BB can support bone formation. From a clinical perspective, the FDA has approved the combination of PDGF-BB and β-tricalcium phosphate for the treatment of periodontal defects (GEM21S®) .

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Platelet Derived Growth Factor is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB is a 24.3 kDa B chain homodimer protein formed by subunits of 109 amino acid residues. Discovery Solutions for CGT GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFbb for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. Recombinant human PDGFbb (HZ-1308) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line.

Within the first week after gene transfer, intimal smooth muscle cell proliferation is present. PDGF BB is expressed by these proliferating smooth muscle cells, suggesting that it can regulate cell proliferation in vivo. Intimal cell proliferation peaked at 1 week and declined 2 weeks after gene transfer. The total number of cells in the intima at 2 to 7 days could not be accounted for by proliferation alone, suggesting that migration of medial smooth muscle cells to the intima may contribute to the intimal lesion. Platelet-derived growth factor-BB (PDGF-BB) is one of the five dimeric proteins belonging to the PDGF family.
As indicated in Fig.1, no complete defect closure was observed in the histological specimens. No substantial bone formation was observed in empty defects; only a thin layer of fibrous soft tissue separated the defect area from the underlying brain tissue. New bone formation was mostly restricted to areas close to the original borders of defects in the control group.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.